Expanded Materials and Methods
ثبت نشده
چکیده
Male Wistar rats (200-300 g) were anesthetized by intraperitoneal injection of sodium pentobarbital (90 mg.kg). The hearts were rapidly removed and placed in a cold (4°C) oxygenated Bicarbonate-buffered solution (in mM): NaCl 125; KCl 5; MgSO4 2; KH2PO4 2; NaHCO3 25; glucose 10. They were cannulated through the aorta on a Langendorff column and retrogradely perfused at a constant flow and at 37°C, with the following solutions: (1) well-oxygenated Bicarbonate-buffered solution for 1-2 minutes in order to wash out the blood, (2) same solution containing 0.06% collagenase (type II, Worthington) and 0.03% hyaluronidase (type IV-S, Sigma) for 20-45 minutes, (3) oxygenated modified KB medium for 2 minutes to stop the dissociation (in mM): K-glutamate 90; KH2PO4 30; MgCl2 1; pyruvic acid 5; taurine 20; glucose 20; HEPES 10 (pH adjusted to 7.2 with KOH). The hearts were then removed from the column, and atria were separated from ventricles. Atria and ventricles were cut into small pieces that were mechanically dissociated into single cells. Isolated myocytes were stored at 4°C in modified KB medium until use (within 8 hours).
منابع مشابه
Evaluation of the Effects of Crushed and Expanded Waste Glass Aggregates on the Material Properties of Lightweight Concrete Using Image-Based Approaches
Recently, the recycling of waste glass has become a worldwide issue in the reduction of waste and energy consumption. Waste glass can be utilized in construction materials, and understanding its effects on material properties is crucial in developing advanced materials. In this study, recycled crushed and expanded glasses are used as lightweight aggregates for concrete, and their relation to th...
متن کاملDifferntiation of Mesenchymal Stem Cells From Cord Blood to Osteblastic Cells
Purpose: This study was carried out to isolate mesenchymal stem cells from full-term cord blood and to differentiate these cells into osteoblastic cells. Materials and Methods: It this study, mesenchymal stem cells were isolated from cord blood and expanded by being cultured in the flask several times. First, the cord blood monoeuc1earcells were separated by ficoll - Hypac before they were cul...
متن کاملFirst-principles based modelling of ferroelectrics
The application of first-principles computational methods to the study of ferroelectric perovskites has greatly expanded our theoretical understanding of this important class of materials. These methods have most recently been applied to an increasingly wide range of ferroelectric materials, with special attention to their lattice dynamics, phase transitions, and dielectric and piezoelectric pr...
متن کاملNanofiber Expansion of Umbilical Cord Blood Hematopoietic Stem Cells
Background The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. Materials and Methods CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads) subsequently, flowcytometry method was done to asses...
متن کاملMesenchymal Stem Cell Purification from the Articular Cartilage Cell Culture
Objective Articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. On the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. Little is known about the presence of mesenchymal stem cells in articular cartilage tissue. This subject is investigated in the present study. Materials and Methods Artic...
متن کامل